Golgappa.net | Golgappa.org | BagIndia.net | BodyIndia.Com | CabIndia.net | CarsBikes.net | CarsBikes.org | CashIndia.net | ConsumerIndia.net | CookingIndia.net | DataIndia.net | DealIndia.net | EmailIndia.net | FirstTablet.com | FirstTourist.com | ForsaleIndia.net | IndiaBody.Com | IndiaCab.net | IndiaCash.net | IndiaModel.net | KidForum.net | OfficeIndia.net | PaysIndia.com | RestaurantIndia.net | RestaurantsIndia.net | SaleForum.net | SellForum.net | SoldIndia.com | StarIndia.net | TomatoCab.com | TomatoCabs.com | TownIndia.com
Interested to Buy Any Domain ? << Click Here >> for more details...


In the method validation proceedure,lod & loq calculation we use the formulae for LOD=3.3*SD/slope and for LOQ=10*SD/slope,why we use 3.3 & 10 in the formulae?



In the method validation proceedure,lod & loq calculation we use the formulae for LOD=3.3*SD/slo..

Answer / veeru01


LOD :- LOD is measured 10 times independent sample of Blank
and Student-t test of 10 times, Degree of freedom 9 (n-1)at
99% confidence label is 3.25 as per Student chart. Also
Ratio of S/N =3:1
LOQ:- LOD is regarded as the lower limit for precise
quantitative Measurement . LOQ defined as concentration
which would a produce a signal 10-12 time. At this point
standard deviation is small enough (Approximately 10-15%) so
that quantified value can be deemed reliable. Also Ratio
S/N= 10:1

Is This Answer Correct ?    7 Yes 3 No

Post New Answer

More Analytical Chemistry Interview Questions

What is the difference between BD

1 Answers  


If compound Pka is 5 then which buffer you will select ?

5 Answers   Avra, GVK,


What is the main difference between ODS1 and ODS2 Hplc column.

0 Answers   Indoco Remedies,


When we calculate Impurity against impurity std.solution and when we calculate impurity against diluted standard with applying RRF?

2 Answers   Torrent Pharma,


what is poarimeter, why it is used in pharma,and explain briefly about polarimeter?

3 Answers   GVK,


In HPLC calibration, caffeine is used as primary standard for wave length calibration due to caffeine is having dual maxima at 273 & 205 nm and one minima at 245 nm. Any body can give reference of these details from any pharmacopeia (with chapter no.) or any other authentic guideline?

0 Answers  


why we get negative values in uv when i placed sample which has some concetration but not in standard

1 Answers   Nirma,


Why are EDTA used to etimation of Ca,Mg etc, in Water analysis?

3 Answers   Reddy Labs,


Why sulfuric acid is used in sulphated ash / residue on ignition test.

9 Answers   CS Pharmaceuticals, Dr Reddys, GVK, HPLC, Micro Labs, Samrudh, Watson Pharmaceuticals, Wings Customer Care,


If combination product how require to identify which imp is of which api?

0 Answers  


What is the importance of rrf in rs by HPLC?

1 Answers  


What is the calibration procedure for Malvern MS2000 particle size analyzer and acceptance criteria?

2 Answers   Reddy Labs,


Categories
  • Organic Chemistry Interview Questions Organic Chemistry (342)
  • Inorganic Chemistry Interview Questions Inorganic Chemistry (123)
  • Analytical Chemistry Interview Questions Analytical Chemistry (1387)
  • Physical Chemistry Interview Questions Physical Chemistry (62)
  • General Chemistry Interview Questions General Chemistry (478)
  • Chemistry AllOther Interview Questions Chemistry AllOther (190)