In the method validation proceedure,lod & loq calculation we use the formulae for LOD=3.3*SD/slope and for LOQ=10*SD/slope,why we use 3.3 & 10 in the formulae?
LOD :- LOD is measured 10 times independent sample of Blank
and Student-t test of 10 times, Degree of freedom 9 (n-1)at
99% confidence label is 3.25 as per Student chart. Also
Ratio of S/N =3:1
LOQ:- LOD is regarded as the lower limit for precise
quantitative Measurement . LOQ defined as concentration
which would a produce a signal 10-12 time. At this point
standard deviation is small enough (Approximately 10-15%) so
that quantified value can be deemed reliable. Also Ratio
S/N= 10:1
| Is This Answer Correct ? | 7 Yes | 3 No |
What is the difference between BD
If compound Pka is 5 then which buffer you will select ?
What is the main difference between ODS1 and ODS2 Hplc column.
When we calculate Impurity against impurity std.solution and when we calculate impurity against diluted standard with applying RRF?
what is poarimeter, why it is used in pharma,and explain briefly about polarimeter?
In HPLC calibration, caffeine is used as primary standard for wave length calibration due to caffeine is having dual maxima at 273 & 205 nm and one minima at 245 nm. Any body can give reference of these details from any pharmacopeia (with chapter no.) or any other authentic guideline?
why we get negative values in uv when i placed sample which has some concetration but not in standard
Why are EDTA used to etimation of Ca,Mg etc, in Water analysis?
Why sulfuric acid is used in sulphated ash / residue on ignition test.
9 Answers CS Pharmaceuticals, Dr Reddys, GVK, HPLC, Micro Labs, Samrudh, Watson Pharmaceuticals, Wings Customer Care,
If combination product how require to identify which imp is of which api?
What is the importance of rrf in rs by HPLC?
What is the calibration procedure for Malvern MS2000 particle size analyzer and acceptance criteria?