How to know HPLC column performance?

Answers were Sorted based on User's Feedback



How to know HPLC column performance?..

Answer / vijay muley

1) check the back pressure,
2) check Theoretical plates,
3) system suitability as per test requirement e.g. tailing
factor, symmetry, RRT, resolution etc.
all three parameters shows columns performance.

Is This Answer Correct ?    20 Yes 0 No

How to know HPLC column performance?..

Answer / darose

check for the number of plates that it maintains stady all
over the run , check the SST at avery run that cpmplies the
acceptance criterias of the method.

Is This Answer Correct ?    5 Yes 2 No

How to know HPLC column performance?..

Answer / venkat reddy

checked the column ressure
check theoretical plates

asymmentry RRT Resolution tailing factor compound method analysis

Is This Answer Correct ?    0 Yes 0 No

How to know HPLC column performance?..

Answer / deepak

To check theoretical plates,peak shape and resolution means sst of method.

Is This Answer Correct ?    0 Yes 0 No

Post New Answer

More Analytical Chemistry Interview Questions

The boiling point of 1,3 butadiene is -4.4 DC & the boiling point of Pentane is 36.1 DC. Then how come Pentane elute before 1,3 BD in GC analysis?

1 Answers  


If mass balance is not passing in forced degradation in method validation that method is suitable or not?

1 Answers   Aurobindo, Dr Reddys, Reddy Labs, Wockhardt,


What is the difference between chiral cel OD-H & OD-RH ?

3 Answers   Aptuit Laurus,


How to develop the method when we have very low amount of impurity?

2 Answers  


why sucrose not used in polarimeter calibration?

2 Answers   Cipla,






What is aggregate and fragments in SEC?

0 Answers  


what is RI detector Cut-off Value

1 Answers   Mylan,


analytical method validation require to with respect to release specification or shelf life specification?

0 Answers  


what is MCG dilution in HPLC & GC analysis

1 Answers   Amoli Organics, Cipla,


why we are using 6 dosage units in dissolution, and why we are using 6 tablets in disintegation test.is there any scientific reason beyond 6?

1 Answers   ABC, Alembic, IPCA,


IN DISSOLUTION S1 STAGE 1 BOWL GOT 52%(Q POINT IS 80%)WHAT WE HAVE DO

9 Answers  


How much carbon loading in nomalphase columns?

5 Answers  


Categories
  • Organic Chemistry Interview Questions Organic Chemistry (342)
  • Inorganic Chemistry Interview Questions Inorganic Chemistry (123)
  • Analytical Chemistry Interview Questions Analytical Chemistry (1387)
  • Physical Chemistry Interview Questions Physical Chemistry (62)
  • General Chemistry Interview Questions General Chemistry (478)
  • Chemistry AllOther Interview Questions Chemistry AllOther (190)