How to know HPLC column performance?
Answer Posted / vijay muley
1) check the back pressure,
2) check Theoretical plates,
3) system suitability as per test requirement e.g. tailing
factor, symmetry, RRT, resolution etc.
all three parameters shows columns performance.
| Is This Answer Correct ? | 20 Yes | 0 No |
Post New Answer View All Answers
1.What is the difference between method validation and method verification 2.Which guidelines proposed to method transfer
Which parameter require to do for analytical method equivalency?
Why we select scan range from higer wavelength to lower wavelength in uv visible spectroscopy and ftir spectroscopy ? .
In which situation we require to analytical method validation of excipient?
how you determine moist.of NaOH sol or NaOH flacks
How they found 1mL of K.F reagent is equivalent to 5mg of water and if we change the composition of K.F reagent, is it can neutralize more amount of water?
mahagenco syllabus for the post of chemist
what is mean by peak intigrity?
What is the acceptance criteria for moisture balance when calibrated with sodium sulfate
In HPLC Calibration, On which basis RSD Limit of noise test is fixed (NMT 33.0 % )
why glutent are detected in the rice cereal baby food product even manufacturer claimed that they are using rice and milk only?we have using ELISA to do the test,and rice supposed not containing any glutent,rite?We already repeat the test so many times and it still detected.just wondering where the glutent came from?
if your product is soluble in 0.1n hcl and water then which you choose as media from these 2 media?
what are risk assement in the analytical qbd?
How can I develope method of dissolution by HPLC OR UV
In HPLC calibration, caffeine is used as primary standard for wave length calibration due to caffeine is having dual maxima at 273 & 205 nm and one minima at 245 nm. Any body can give reference of these details from any pharmacopeia (with chapter no.) or any other authentic guideline?