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how can we calculate void volume?give its importance in brief
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Answer / sekhar.kranam
by injecting Uracil it will elute below 2 minutes
elution of un retained peak
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Column selection in HPLC method development?
What is the difference between potentiometry assay and HPLc assay?
2 Answers Apotex, Aurobindo, Divis Laboratories, Torrent Power,
inhouse product is in capsule form in combination and RLD is in tablet form then can we proceed for multimedia CDP? in inhouse capsule product disso is paddle with sinker in release media is there then RLD product in tablet form then with same as paddle with sinker we can proceed n.a.?
[3/28, 20:52] Manoj P Venkatpurwar: how many impurities require to inject in assay specificity that how we can find out? and in Rs also how?
What is the reason for negative peak in HPLC while using UV detector.
In HPLC Chromatographic separation Resolution equation is Rs = 1.18 (tR2 –tR1)/wh1+Wh2, Where, tR2 and tR1 is run time of eluent ratios of the distances from the point of application to the centers of the spots and the distance travelled by the solvent front from the point of application (retardation factor), and Wh1and Wh2 , is peak width of at half height. What is 1.18 ?
what is the difference between polarimeter lamp and ir lamp
In gas chromatographic analysis sometimes we use headspace, why it is required instead of manually injection?
In GC and HPLC ,how we can decide which of following method will be suitable or use for quantitation of chromatogram at the time of method development. 1.Area Normalisation 2.External standard method 3.Internal standard method
verification of wave number why you are use in IR calibration
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What is reaction takes place when water content analysis is done
Organic Chemistry 
