Answer / satheesh kumar

Columns selection is main role for the separation of
compounds in method development.

1)Compounds solubility to be check
2)pKa of the compound

we know if the compound is acidic,fix pH nearer to pKa(+/-2)
Starts with ODS Column check the Compound RT,peak shape and
tailing criteria.
Generally HPLC Columns work between pH 2-8.If too acidic or
basic then columns packing will dissociate,
Some special columns are there to work in higher pH 8 to
namely X-Terra RP 18,Phenomenx Gemini C18.

Ensure what pH to be works for the concerned compound
select the columns accordingly

In which cases factor of KF Reaget got higher side

2 Answers  

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give me method of calculating co relation of coefficient for HPLC,SOR & ANY OTHER

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why 1.2%kcl solution is using in limit os straylight?and why this test used for uv calibration?

1 Answers   Graviti Pharmaceuticals, Reddy Labs, Vimta Labs,

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Why do we use 500ppb sucrose and 500ppb 1,4 benzoquinone in TOC calibration?

2 Answers   Vardhman,

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U have 4 strengths have different coating meterial which one u chose to do forced degradation stady in validation

1 Answers   Reddy Labs,

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Can we do the MC analysis of a sample which is not completely soluble in the medium choosed.

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Describe your field experience sample type collected,sample techniques,field measurements taken and equipment used?

0 Answers   Apex, BNM, Ranbaxy,

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How much carbon loading in nomalphase columns?

5 Answers  

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what is the definition of limit of stray light in UV calibration

1 Answers   FDC, Zydus Cadila,

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Why does boiler drum ph maintain from 9.5 to 10.2

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iam using ph instrument lab india make.3ponit and 5point.in calibration time 3point slope will come 98.89and 96.87.that limit is 85 to 105.what basis slope will come.any calucalation between them.5point wiicome slope 99.89?why 3p0int came 2 values

1 Answers  

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