What is the reason for negative peak in HPLC while using UV detector.
- 2 Answers
- 14736 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / ravi mishra
If your diluent is more polar than mobile phase then such negative peak appear.
| Is This Answer Correct ? | 11 Yes | 1 No |
Answer / hemesh
in UV detector cell precipitate might be formed.
it should be cleaned with hot water or diluent in which it dissolve.
| Is This Answer Correct ? | 1 Yes | 3 No |
Why Holium cholorate used in UV Spectro photometer?
What is the use of tailing factor ?
Please do not join if u got offer in oncotherapies ltd (STRIDESARCOLAB)especially Mr.Mahesh Bhatt team because job is not secure his team and doesn't have confidence on him becareful this offer?
what is the importance of peak purity in HPLC and how we can calculate through manul(not software)?
What is the importance of sulphated ash test?
How can you prepare 0.1% Phosphate buffer solution?
What is cleaning validation of rinse and swabe and its limit with caculation formula.
Please give idea about method development for cleaning method and how maco level establish
What is difference between Primary and secodary reference standard?
what is peak purity and its formula
what are the standard methods for HPLC?
how v can identify functional groups by wet techniques?
Organic Chemistry 
