Golgappa.net | Golgappa.org | BagIndia.net | BodyIndia.Com | CabIndia.net | CarsBikes.net | CarsBikes.org | CashIndia.net | ConsumerIndia.net | CookingIndia.net | DataIndia.net | DealIndia.net | EmailIndia.net | FirstTablet.com | FirstTourist.com | ForsaleIndia.net | IndiaBody.Com | IndiaCab.net | IndiaCash.net | IndiaModel.net | KidForum.net | OfficeIndia.net | PaysIndia.com | RestaurantIndia.net | RestaurantsIndia.net | SaleForum.net | SellForum.net | SoldIndia.com | StarIndia.net | TomatoCab.com | TomatoCabs.com | TownIndia.com
Interested to Buy Any Domain ? << Click Here >> for more details...

why buffer is added in solvent system in HPLC ??

Answer Posted / msreddyvelagala

in solute we may have different products,all these cannot elute at same concetration of mobilephase in short time.solvent is polar,to get the eluates faster we have tochange the mobilephase composition(i.e polarity to be increased up to certain time intervel)

Is This Answer Correct ?    3 Yes 1 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

In Assy by potentio metry titration method how select MET U ,DET U, SET U,

2070


Why to use propyl paraben in HPLC gradient calibration

7319


CAN WE DETERMINE THE PEAK PURITY IN GAS CHROMATOGRAPHY ANALYSIS?

1149


in which situation require to change rs specification?

702


why sre you used Potassium hydrogen phthalate in standarisation of 1N NaOH and 0.1 N Perchloric Acid?

9693


why require the ph, buffer during hplc mobile phase?

816


inhouse product is in capsule form in combination and RLD is in tablet form then can we proceed for multimedia CDP? in inhouse capsule product disso is paddle with sinker in release media is there then RLD product in tablet form then with same as paddle with sinker we can proceed n.a.?

660


What is related substance by HPLC impurity limits as per USP?

1031


in hplc chromatogram started from left to right and in uv spectrum started from left to right

889


what is the structure of DOWEX-50

5451


What are usp limits for theoritical plats,resoution,tailing factor,peak to valley ratio

1850


why salisylic acid not using now days for disdolution calibration?

713


Tell me something about Vitamin A test method by HPLC

2627


why we should take dst factor for below 1%moisture samples

2612


Why do we use KMnO4 in the test of control of obsorbance ? and why do we take specific quantity i.e 57-63mg?

2775