why buffer is added in solvent system in HPLC ??
Answer Posted / msreddyvelagala
in solute we may have different products,all these cannot elute at same concetration of mobilephase in short time.solvent is polar,to get the eluates faster we have tochange the mobilephase composition(i.e polarity to be increased up to certain time intervel)
| Is This Answer Correct ? | 3 Yes | 1 No |
Post New Answer View All Answers
4. Describe the operation of the Craig apparatus. Chapter.3 Equilibrium Processes in Separations
How will u perform degradation study in detectors other thann PDA in HPLC Methodology
Related substance method equivalency on control sample or spiked sample?
function of detecter in hplc ,gc and spectroscopy? function of carrier gas in gc?
how a particular wavelength can be different for a particular compund while analysing by uv and by HPLC.
How to decide assay range for non pharmacopeial API analysis by HPLC? Could you give me any reference for same e.g Guidelines or Paper publications?
1.What is the difference between method validation and method verification 2.Which guidelines proposed to method transfer
how to calibrate hplc & gc
for heavy metal test lead used in which form pure form or any other form for preparation of lead standard sol
What is control room temperature and which guide line says?
What is the formula to determine the concentration of M of a solution given the % transmittance? Use %T = 43.7 as an example.
In which situation we require to prepare the standard solution from sample in Related substance method?
For titration in anhydrous media with perchloric acide, if lack of titrator, Which indicator is been used for replacement. How calculate pH of test solution to choose suitable indicator?
What is shaking level in GC?
what is lod and loq ?,why use k2cr2o7 , kcl h2so4 in uv calibration ?,why use benzophenone & caffene acetone in hplc calibration ?,what is leading peak in hplc ?why we do the calibration of limit of stry light in hplc & uv ?