Interested to Buy Any Domain ? << Click Here >> for more details...
today i am doing calibration of HPLC. when i inject the
blank it was going on -ve.(bleow then zero) why?
- 3 Answers
- 10869 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / b.gangatharan
This is due to improper sonication of mobile phase and improper stabilisation of column.
Regards,
B.Gangatharan
| Is This Answer Correct ? | 7 Yes | 1 No |
Answer / sudheer kumar m
may be mobile phase was not stable in that particular
method. so condition the column with mobile phase and re inject.
| Is This Answer Correct ? | 2 Yes | 2 No |
Negative peak simply indicate that your mobile phase
absorbs UV light.So use only HPLC grade solvents.Even with
HPLC grade solvents you get the negative peaks you system
might not be working properly.
Regards
M.KIRAN REDDY
| Is This Answer Correct ? | 2 Yes | 7 No |
how can we decide the limit of cleaning validation? What is the criteria for it?
During Validation study, Recovery is passing at all levels but Linearity is failing. Justify?
1 Answers Mylan, Torrent Pharma,
IN DISSOLUTION WE ARE USING A LETTER 'Q' WHAT IS MENT BY Q
5 Answers Reddy Labs, Torrent Pharma,
how to develop the icp ms method? Application of icp ms?
Can you please give a suitable definition and an example for understanding the words purity and potency.
6 Answers Cipla, Malladi Drugs and Pharmaceuticals, Reddy Labs,
why 0.02% solution toluene &noHexane using in uv calibration?
1 Answers Graviti Pharmaceuticals, Orbit Pharma, Ranbaxy, Vimta Labs,
%lod always grater than %water content for same product??
What is (Q) point dissolution?
Wath is the differenc between two column HPLC wich have the samme support , lenth , pore size, etc.. But different name .for exemple : Spherisorb / lichrosorb
why IR graph or spectra comes down ? Any reason
how many types of balance calibration test?
What is heavy water
Organic Chemistry 
