Answer / b.gangatharan

This is due to improper sonication of mobile phase and improper stabilisation of column.
Regards,
B.Gangatharan

Answer / sudheer kumar m

may be mobile phase was not stable in that particular
method. so condition the column with mobile phase and re inject.

Answer / m.kiran reddy

Negative peak simply indicate that your mobile phase
absorbs UV light.So use only HPLC grade solvents.Even with
HPLC grade solvents you get the negative peaks you system
might not be working properly.

Regards
M.KIRAN REDDY

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