Golgappa.net | Golgappa.org | BagIndia.net | BodyIndia.Com | CabIndia.net | CarsBikes.net | CarsBikes.org | CashIndia.net | ConsumerIndia.net | CookingIndia.net | DataIndia.net | DealIndia.net | EmailIndia.net | FirstTablet.com | FirstTourist.com | ForsaleIndia.net | IndiaBody.Com | IndiaCab.net | IndiaCash.net | IndiaModel.net | KidForum.net | OfficeIndia.net | PaysIndia.com | RestaurantIndia.net | RestaurantsIndia.net | SaleForum.net | SellForum.net | SoldIndia.com | StarIndia.net | TomatoCab.com | TomatoCabs.com | TownIndia.com
Interested to Buy Any Domain ? << Click Here >> for more details...

today i am doing calibration of HPLC. when i inject the
blank it was going on -ve.(bleow then zero) why?

Answer Posted / m.kiran reddy

Negative peak simply indicate that your mobile phase
absorbs UV light.So use only HPLC grade solvents.Even with
HPLC grade solvents you get the negative peaks you system
might not be working properly.

Regards
M.KIRAN REDDY

Is This Answer Correct ?    2 Yes 7 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

For titration in anhydrous media with perchloric acide, if lack of titrator, Which indicator is been used for replacement. How calculate pH of test solution to choose suitable indicator?

2306


give clarity of linearity and range in method validation

2413


What is rs test why we are performing rs test?

1851


how many types of balance calibration test?

2295


Which type of column should i use to check the purity of high molecular weight protein using HPLC reverse phase column chromatography? Hi everyone. I wanna to check the purity of high molecular weight protein (collagen) with MW of ~130 kDa using a HPLC. I know C18

1908


What is stray light in uv what are units for stray light

1635


how you establish the LOQ?

780


A ha tc is actual defination of retention time?

1612


in which situation ion pair require to use?

801


2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.

2212


what is turbidimetric titration?Give 2 examples.

2651


Why sodium hydroxide used for maintain pH of phosphate buffer

2402


what is the principle of UV Vis spectroscopy, AAS, ICP OES,ICPAES, ICP-MS and FTIR

9241


What is split ratio in Gc? Splitless? how requirr to select?

872


How can we calculate "confidence interval" in analytical method validation? Pl. explain with example.

4254