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how to hplc calibrated
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HPLC is calibrated by the following parameters:
1) Pump calibration
2) Detector Linearity
3)Injector Precision and carryover test
4) Wavelength accuracy by using caffeine and pyrene solution
5) Determine the column oven temp. and sample compartment temp
6) Gradient test
7) Detector noise
| Is This Answer Correct ? | 128 Yes | 13 No |
Answer / ajinkya p.
In HPLC there must calibrations for Pump, Detector,injector
Pump :- Should calibrated by checking flow accuracy test, Pressure holding test.
Detector :- Should calibrated by checking Linearity test, Detector wavelength accuracy test
Auto sampler :- Should calibrated by Injector Accuracy test,
Carryover test, Injector precision test, carry over test
| Is This Answer Correct ? | 48 Yes | 2 No |
Answer / kanakadurga
calibration parameters are
1.Flow accuracy
2Temperature accuracy
3Wave length accuracy by using caffeine and erbium perchlorate
4Linearity of detector and injector
5.Carry over test
6.Drift and noice
| Is This Answer Correct ? | 37 Yes | 6 No |
Answer / b.ganesh
calibration parameters are
1.Flow accuracy
2Temperature accuracy
3Wave length accuracy by using caffeine and erbium perchlorate
4Linearity of detector and injector
5.Carry over test
6.Drift and noice
7.Gradient progromme accuracy
8.injector precision
| Is This Answer Correct ? | 3 Yes | 1 No |
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