Interested to Buy Any Domain ? << Click Here >> for more details...
how to hplc calibrated
- 4 Answers
- 52691 Views
- Dr Reddys, Glochem, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
HPLC is calibrated by the following parameters:
1) Pump calibration
2) Detector Linearity
3)Injector Precision and carryover test
4) Wavelength accuracy by using caffeine and pyrene solution
5) Determine the column oven temp. and sample compartment temp
6) Gradient test
7) Detector noise
| Is This Answer Correct ? | 128 Yes | 13 No |
Answer / ajinkya p.
In HPLC there must calibrations for Pump, Detector,injector
Pump :- Should calibrated by checking flow accuracy test, Pressure holding test.
Detector :- Should calibrated by checking Linearity test, Detector wavelength accuracy test
Auto sampler :- Should calibrated by Injector Accuracy test,
Carryover test, Injector precision test, carry over test
| Is This Answer Correct ? | 48 Yes | 2 No |
Answer / kanakadurga
calibration parameters are
1.Flow accuracy
2Temperature accuracy
3Wave length accuracy by using caffeine and erbium perchlorate
4Linearity of detector and injector
5.Carry over test
6.Drift and noice
| Is This Answer Correct ? | 37 Yes | 6 No |
Answer / b.ganesh
calibration parameters are
1.Flow accuracy
2Temperature accuracy
3Wave length accuracy by using caffeine and erbium perchlorate
4Linearity of detector and injector
5.Carry over test
6.Drift and noice
7.Gradient progromme accuracy
8.injector precision
| Is This Answer Correct ? | 3 Yes | 1 No |
When i inject amines and acid on HPLC then which peak will elut first?
How we will choose pH of a mobile phase if analyte pKa value is 4
what is the Bracketing & Matrixing.
In Analytical method development related substance method when did follow w/w method when did follow area normalisation method
2 Answers Dr Reddys, Sun Pharma,
for which type of applications gas chromatography is used?
plz tall me that any pharmacopeia and guideline is par meted to use 1.68,4.01,7.00,9.00,12.45 buffer for ph meter calibration
Many times I don't got a caffeine peak in calibration of hplc using guard column ❓
How can we calculate "confidence interval" in analytical method validation? Pl. explain with example.
why we use sodium salicylate in uv light calibration at TLC chamber
1 Answers Aurobindo, Lee Pharma,
why water factor is near about 5.0 in kf titration?
3 Answers Claris Lifesciences, Indchemie Health Specialities,
in gas chromatography what is the difference between gas flow rate and average linear velocity ?
What should be the calibration procedure for wavelength accuracy in case of HPLC?
Organic Chemistry 
