How did you conclude that when stressing a compond, all
degradation products have eluted in chromatogram?
- 7 Answers
- 9967 Views
- Matrix, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / s.elumalai
The main aim of degradation study is to separate know
impurities in the case when we do not have know impurities
or to generate impurities from the sample by degradation.
Obviously one should know the process related impurities or
the degradation pathways. From peak purity by PDA or Mass
analysis one can conclude the purity of each peak eluting,
beyond that mass balance also account for peaks.If mass is
not balanced then one can conclude that some of the compound
are not eluted or may not have UV absorption.
| Is This Answer Correct ? | 7 Yes | 0 No |
Answer / hemant
Method should be stability indicating & should give mass
balance
| Is This Answer Correct ? | 3 Yes | 0 No |
Answer / naga
in specificity,by mass balance study we can confirm all
impurities are eluted in specified run time.
Mass balance = Assay of main peak+total net degradation
from this we can conclude what r the degradants r there all
r eluted the desired runtime.
Mass balance limit NLT 95%
| Is This Answer Correct ? | 3 Yes | 0 No |
Answer / m.sivalingeswararao
By calculating mass balance.It should be equal to or nearer to 100%.%Decrease in mass balance indicates the % of Impurities not detected or not eluted. In some cases Degraded Impurities may not be eluted with in specified run time,in such cases they may elute in the next run ,hence it should be advisable to increase the run time.
| Is This Answer Correct ? | 1 Yes | 0 No |
Answer / pramod bhosale
In a dregradation process there should no effect of main
drug when degradant peak elutes in degradation.
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / babu.s
when we stressing a compound,all degradation products are may eluted or may not be eluted in chromatogrm.Due to stressing the form of drug my be changed to some extent. in that cases, the changed form is identified by pXRD/dsc.so better go TO UV/IR FOR BETTER RESULT.
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / divya
degrading produts are considered as impurities and small in
size so they elute fast.
| Is This Answer Correct ? | 1 Yes | 13 No |
Why we have to use KBr pellet technology ?
What is the difference between Discriminating media and DPDM(Dissolution Profile with Different Media)
If we have 5 strength which is not dose proportinate and excipients also diffrent in each strength then how we can proceed for Force degradation? and excipient are same but not dose propotinate the how FD?
What is normality
How you develop a method in HPLC?
0 Answers CU, Perrigo, Wockhardt,
what are the primary standards of redox titrations?Name any two.
what is stability study ? why it is needed and how itis done
Which Pharmacopiea follow calibration of HPLC,GC
why plane of polarized light rotated by opticaly active compunds?
As per ICH related substances stability trend limit from initial to shelf life
0 Answers Medley Pharmaceuticals, Micro Labs,
what are the basic requirement for the WHO audit?
How to prepare 1000ppm methylene blue solution
Organic Chemistry 
