how to hplc calibrated

Answer Posted / ajinkya p.

In HPLC there must calibrations for Pump, Detector,injector
Pump :- Should calibrated by checking flow accuracy test, Pressure holding test.
Detector :- Should calibrated by checking Linearity test, Detector wavelength accuracy test
Auto sampler :- Should calibrated by Injector Accuracy test,
Carryover test, Injector precision test, carry over test

Is This Answer Correct ?    48 Yes 2 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

How to decide assay range for non pharmacopeial API analysis by HPLC? Could you give me any reference for same e.g Guidelines or Paper publications?

2860


how require to select dissolution media? what is discrimination?

309


How require to develop GC method? how to select diluent, GAS, column selection and other chromatographic conditions?

272


Why six unit used for precision?

798


in stress study if your api not soluble in hcl naoh h2o2 then what require to do?

325






what is mean by 40 in the dissolution basket mesh size?

287


Related substance method equivalency on control sample or spiked sample?

299


what is mean by peak intigrity?

337


For titration in anhydrous media with perchloric acide, if lack of titrator, Which indicator is been used for replacement. How calculate pH of test solution to choose suitable indicator?

1786


in which situation require to take incident in validation?

748


Why to use propyl paraben in HPLC gradient calibration

6074


In rs method development when we are going area normalization method to dilute standard method?

943


Principle of single pan analytical balance

4598


Why acetonitrile and water are used as extraction solvent when analysis melamine? I thought they are miscible and won't be able to separate...

1216


What is third generation HPLC Columns?

1507