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what r the reasons to get peak telling?

Answers were Sorted based on User's Feedback



what r the reasons to get peak telling?..

Answer / ravi kumar

due to the presence of residual silanols on the reverse
phase column which interacts with acids or bases (mostly
bases)cause them to tail

Is This Answer Correct ?    19 Yes 1 No

what r the reasons to get peak telling?..

Answer / nageswar rao

the following parametrs are reasons
1.silinol interaction of basic amines
2.adsorption of acidic compounds
3.mass overloaded
4.injecting sample in worng solvent significantly stroger
than mobile phase

Is This Answer Correct ?    15 Yes 1 No

what r the reasons to get peak telling?..

Answer / nagesh.v@sai advantium.com

Due to column efficiency Decreaced

Is This Answer Correct ?    11 Yes 2 No

what r the reasons to get peak telling?..

Answer / subash

The following reasons are causes of peak tailing
1.Injecting the sample in a solvent significantly stroger
than the mobile phase
2.Interaction of stationary phase silanols with basic
analyte
3.Adsorption of acidic compounds on the stationary phase
4.Sample mass overloaded
5.A void in the column packing bed

Is This Answer Correct ?    5 Yes 0 No

what r the reasons to get peak telling?..

Answer / t.venkata reddy

below reasons causes peak tailing
1)Analytical column contaminated
2)Mobile phase contamination/deterioation
3)unswept dead volume
4)basic substance-silanol interation.

Is This Answer Correct ?    2 Yes 0 No

what r the reasons to get peak telling?..

Answer / palabindela

column over loaded or high injection volume

Is This Answer Correct ?    0 Yes 2 No

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