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what r the reasons to get peak telling?

Answer Posted / subash

The following reasons are causes of peak tailing
1.Injecting the sample in a solvent significantly stroger
than the mobile phase
2.Interaction of stationary phase silanols with basic
analyte
3.Adsorption of acidic compounds on the stationary phase
4.Sample mass overloaded
5.A void in the column packing bed

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