Answer / ashok babu jha

Procedure:
30 mL methanol +3 g imidazole +1g N-
Ethylmaleinimide . Titrte to dryness , add approx.03 g
sample from a dry syringe without needle , allow to react
under stirring for 5 min and then titrate the water content
with KF reagent.Ethylmaleinimide form covalent bonds with
sulfhydryl groups at pH 6.5-7.5 .

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