In the test for control of absorbance in UV calibration why
do we use only potassium dichromate and what is purpose of
taking a specified amount of 57.0-63.0mg?
Answers were Sorted based on User's Feedback
Answer / bhagya lakshmi.k
It will show max absorbance in Ultraviolet region (200-
400nm) (i.e) at 235,257,313,350 nm
| Is This Answer Correct ? | 53 Yes | 4 No |
Answer / sunil.m.kulkarni ( avon/arch p
Potassium Dichromate - UV Absorbance and Linearity
Product Description:
The use of potassium dichromate solvated in dilute
perchloric acid or suplhuric acid is an established and
well recognised method for the validation of the absorbance
scale and linearity of a spectrophotometer in the UV
region.
When prepared in 0.001M perchloric /Suplhuric acid,
potassium dichromate solution gives a spectral scan
containing characteristic peaks at 257 nm and 350 nm, and
troughs at 235 nm and 313 nm. Within the concentration
range 20-100 mg/l, if the absorbance scale of a narrow SBW
(< 2 nm) spectrophotometer is linear, the apparent
absorbances of a series of concentrations will be a linear
function of concentration.
Note: Primary standard ( Extra pure potassium dichromte
after drying to be used for calibration ) 57-63mg in -
1000ml concentration is perfect concentration for achiving
perfect charecteristic peak and throughs
| Is This Answer Correct ? | 36 Yes | 5 No |
Answer / sagadevan
becoz of photochemical stability,so on prolonged exposure to uv light the absorbance own not change,
| Is This Answer Correct ? | 18 Yes | 4 No |
Answer / devidas
it show the maxima at 235nm,257nm,313nm, and 350nm in UV
region. and it consider as a primary standard which is high
purity
| Is This Answer Correct ? | 18 Yes | 7 No |
Answer / vivek
we are use potassium dichromate for the verification of spectrophotometer because it show maximum absorbtion in uv region...
| Is This Answer Correct ? | 12 Yes | 5 No |
Answer / srinathsagar
UV range is 190-400nm so in this range potassium dichromate with sulfuric acid gives characteristic peaks(At 235,257,313 and 350nm)
| Is This Answer Correct ? | 4 Yes | 1 No |
because it covers uv to visible region i.e. 235nm,257nm,313nm,350nm
| Is This Answer Correct ? | 6 Yes | 4 No |
why using factor 1.00639 in burette calibration of kf
Defination of ZEEMAN EFFECT in NMR ?
How can we determine Moisture content of HI?
is it necessary to do HPLC calibration at wavelength 315nm if we are doing analysis at this wavelangth
What is post run in hplc?
which effect is mainly responsible for chromatographic separation when silica is the statinary phase at TLC? thanks in advance
what is the moisture content of Disodium hydrogen tartrate. how it can be calculted. as 16.5%
In the test for control of absorbance in UV calibration why do we use only potassium dichromate and what is purpose of taking a specified amount of 57.0-63.0mg?
why we can use only 4 and 7 and 9 buffers in pH calibration
What are the differences between GC and HPLC?
If we have 5 strength which is not dose proportinate and excipients also diffrent in each strength then how we can proceed for Force degradation? and excipient are same but not dose propotinate the how FD?
India is. Under which zone as per QF1.?
4 Answers Acebright, Aurobindo, Gland Pharma,