In the test for control of absorbance in UV calibration why
do we use only potassium dichromate and what is purpose of
taking a specified amount of 57.0-63.0mg?
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Answer Posted / sunil.m.kulkarni ( avon/arch p
Potassium Dichromate - UV Absorbance and Linearity
Product Description:
The use of potassium dichromate solvated in dilute
perchloric acid or suplhuric acid is an established and
well recognised method for the validation of the absorbance
scale and linearity of a spectrophotometer in the UV
region.
When prepared in 0.001M perchloric /Suplhuric acid,
potassium dichromate solution gives a spectral scan
containing characteristic peaks at 257 nm and 350 nm, and
troughs at 235 nm and 313 nm. Within the concentration
range 20-100 mg/l, if the absorbance scale of a narrow SBW
(< 2 nm) spectrophotometer is linear, the apparent
absorbances of a series of concentrations will be a linear
function of concentration.
Note: Primary standard ( Extra pure potassium dichromte
after drying to be used for calibration ) 57-63mg in -
1000ml concentration is perfect concentration for achiving
perfect charecteristic peak and throughs
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