Answer / loges

in colourless compond seperatiion each 100ml fraction
collected seperately and using Thinlayer chromatography
check the seperated fraction by using UV lamp

Answer / dipti

i colomn chromatograph, if colorless compound has 2
seperate then, another compound is used which is colled as
coloring compound, has the ability to give color with the
compond has to detect.

Answer / trevor molyneux

There are number of possibilities for identifying the
eluents from the column. Try passing the eluent through a
UV beam if any of the eluents fluoresce. You could also
pass the eluent through a set up with an IR source and
detector or a refractive index measuring instrument. Any
of these alternatives could be used quantitatively.

Answer / k.madhu

WE R SEPERATED with hptlc method and some pigments is
present so i seperate colorlees

WHAT IS USE OF ION PAIR REAGENTS?

3 Answers  

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why we inject sample TWICE in ASSAY by HPLC analysis but in RS once?

4 Answers   Aurobindo, DRL, Neuland,

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if you get peak in blank then what require to do?

0 Answers  

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what are the guidelines for analytical method validations?

0 Answers  

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why RID detector is used for selective materials like Mannitol, Hydroxyethyl Starch and Sorbitol?

5 Answers  

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How do you increase the number of theoretical plates?

1 Answers   Cipla,

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why the sucrose used in calibration of polarimeter

5 Answers   Neuland,

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wt is rsd in and explain

4 Answers   Cadila, Reddy Labs,

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In acid base titration, after complet consumption of HCl by NaOH, addition of next drop of NaOH will give pink colour with phenolpthalein indicator. Why this pink colour appear? Why not blue, black, green or anything elese?

3 Answers  

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why chiral separation?

1 Answers  

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what is the principle involved in determination of moisture content?

3 Answers  

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Sulphated Ash incresed it will effect on product yield?

3 Answers   Biocon, Lupin,

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