Answer / loges

in colourless compond seperatiion each 100ml fraction
collected seperately and using Thinlayer chromatography
check the seperated fraction by using UV lamp

Answer / dipti

i colomn chromatograph, if colorless compound has 2
seperate then, another compound is used which is colled as
coloring compound, has the ability to give color with the
compond has to detect.

Answer / trevor molyneux

There are number of possibilities for identifying the
eluents from the column. Try passing the eluent through a
UV beam if any of the eluents fluoresce. You could also
pass the eluent through a set up with an IR source and
detector or a refractive index measuring instrument. Any
of these alternatives could be used quantitatively.

Answer / k.madhu

WE R SEPERATED with hptlc method and some pigments is
present so i seperate colorlees

why results are variation in same samples analysis in gc and hplc ?

2 Answers  

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what is Hilic chromatography?

1 Answers  

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What is mean by peak tailing in case of GC?

5 Answers  

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what v define from IR & NMR spectroscopy ?

2 Answers   Sun Pharma,

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What is peak purity and response factor in chromatographic analysis? Why we required to find out response factor?

2 Answers  

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What is primary & Secondary Standard ? Give Example

17 Answers   Cipla, Lupin,

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Why caffeine as a reference standard used for calibration of HPLC?

11 Answers   Gangwal Chemicals, Orchid,

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How to calculate lamp energy in hplc?

1 Answers  

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what are the different methods of analysing related substances test,what are LOD AND LOQ .HOW TO REPORT IMPURITIES WHEN LOD AND LOQ LIMITS R SPECIFIED

3 Answers   Dr Reddys,

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What is the limit of minimum area in in hplc RS anlysis

1 Answers   Lupin,

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HOW RRF IS CALCULATED IN HPLC

2 Answers  

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What is the difference between known and unknown impurities?

1 Answers  

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