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Why we use Caffeine in hplc calibration

Answers were Sorted based on User's Feedback



Why we use Caffeine in hplc calibration..

Answer / padma

It gives a multiwavelength response with Maxima at 205nm and 273nm and minima at 245nm ,it is stable for long time and easily avialable in market and cheap.It is specially for wave length accuracy since it will scan the whole range of UV

Is This Answer Correct ?    54 Yes 0 No

Why we use Caffeine in hplc calibration..

Answer / venkatesh

Caffeine is thermally stable natural product, Detects on
multi wavelengths like 273,254,etc.. and also not degradable.-

Is This Answer Correct ?    6 Yes 0 No

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