in HPLC injection volume is increase ,R.T will increase ?

Answers were Sorted based on User's Feedback



in HPLC injection volume is increase ,R.T will increase ?..

Answer / b.gangatharan

In hplc area will increase when injection volume is increased.But rt will not increase or change.

Is This Answer Correct ?    27 Yes 1 No

in HPLC injection volume is increase ,R.T will increase ?..

Answer / srikar_22

Increasing sample volume did not show any affect on rt

Is This Answer Correct ?    5 Yes 1 No

in HPLC injection volume is increase ,R.T will increase ?..

Answer / ravi1234

There will be no relation Between Inj Volume and Retention time.If Injection Voulme Increases Area of Peak Changes..Not RT.

Is This Answer Correct ?    2 Yes 0 No

in HPLC injection volume is increase ,R.T will increase ?..

Answer / bindeshwar prasad

Whenever sample concentration will increases there is chance to slight decrease in Rt with respect to lower concentrations Rt.

Is This Answer Correct ?    2 Yes 2 No

in HPLC injection volume is increase ,R.T will increase ?..

Answer / sachin

because of more sample concentration peak will take time to elute.

Is This Answer Correct ?    7 Yes 18 No

Post New Answer

More Analytical Chemistry Interview Questions

Which water can use Hplc analysis

9 Answers   BioGenomics, Dr Reddys, Reddy Labs,


What's the importance of these procedures?

1 Answers  


Sulphated Ash incresed it will effect on product yield?

3 Answers   Biocon, Lupin,


Is it necessary to inject three replicate for accuracy in AMV.

1 Answers   Amoli Organics,


What is meaning of audit trail in hplc

3 Answers   DRL, Lee Pharma, Raks Pharma,






In which cases factor of KF Reaget got higher side

2 Answers  


Why using 0.5% acetone in methanol mobile phase for HPLC Gradient performance check.

2 Answers   DRL,


What is the difference between bulk property detectors and Solute property detectors vivimed labs limited

3 Answers  


why we prefer kbr for ftir than kcl?

5 Answers   Aurobindo,


Why we show ammonia vapours to tlc plate?to which compounds?

1 Answers  


Give me details about dissolution , with name ?

1 Answers   EPCA,


HOw do i calculate the number of theoretical plate on a HPLC chromatography

2 Answers   Laurus,


Categories
  • Organic Chemistry Interview Questions Organic Chemistry (342)
  • Inorganic Chemistry Interview Questions Inorganic Chemistry (123)
  • Analytical Chemistry Interview Questions Analytical Chemistry (1387)
  • Physical Chemistry Interview Questions Physical Chemistry (62)
  • General Chemistry Interview Questions General Chemistry (478)
  • Chemistry AllOther Interview Questions Chemistry AllOther (190)