in HPLC injection volume is increase ,R.T will increase ?

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Question Posted / imam

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in HPLC injection volume is increase ,R.T will increase ?..

Answer / b.gangatharan

In hplc area will increase when injection volume is increased.But rt will not increase or change.

Is This Answer Correct ?

27 Yes

1 No

in HPLC injection volume is increase ,R.T will increase ?..

Answer / srikar_22

Increasing sample volume did not show any affect on rt

Is This Answer Correct ?

5 Yes

1 No

in HPLC injection volume is increase ,R.T will increase ?..

Answer / ravi1234

There will be no relation Between Inj Volume and Retention time.If Injection Voulme Increases Area of Peak Changes..Not RT.

Is This Answer Correct ?

2 Yes

0 No

in HPLC injection volume is increase ,R.T will increase ?..

Answer / bindeshwar prasad

Whenever sample concentration will increases there is chance to slight decrease in Rt with respect to lower concentrations Rt.

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2 Yes

2 No

in HPLC injection volume is increase ,R.T will increase ?..

Answer / sachin

because of more sample concentration peak will take time to elute.

Is This Answer Correct ?

7 Yes

18 No

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