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what is use of acetonitrile compare to methanol in RP-HPLC
Method devlopment
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Answer / sandesh
the uv cut oof value of methanol is 210 nm so we cant use
methanol near about lambda max of 200 nm. so prefer to ACN
| Is This Answer Correct ? | 28 Yes | 4 No |
Answer / balaji
the uv cut oof value of methanol is 210 nm so we cant use
methanol near about lambda max of 200 nm. so prefer to ACN
& ACN is highly polar as compare to Methanol So provide
Better resolution for many compound and it has property to
form hydrogen bond so provide better selectivity.
Methanol viscosity more than the ACN. so ACN easly flow
through column
| Is This Answer Correct ? | 15 Yes | 1 No |
Answer / dharmesh radadiya
ACN is highly polar as compare to Methanol So provide Better
resolution for many compound and it has property to form
hydrogen bond so provide better selectivity.
| Is This Answer Correct ? | 17 Yes | 10 No |
Answer / snehal
ACN is more polar and neutral solvent and has low viscosity than methanol hense shows low back pressure than methanol
| Is This Answer Correct ? | 7 Yes | 3 No |
Answer / guest
strength of elution varies among different organic solvents...among methanol and acetonitrile, acn has higher elution strength than methanol
| Is This Answer Correct ? | 1 Yes | 3 No |
Answer / rohithirpara
Dear Dharmesh
Methanol is very polar than ACN
| Is This Answer Correct ? | 7 Yes | 28 No |
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Organic Chemistry 
