what is use of acetonitrile compare to methanol in RP-HPLC
Method devlopment

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what is use of acetonitrile compare to methanol in RP-HPLC Method devlopment..

Answer / sandesh

the uv cut oof value of methanol is 210 nm so we cant use
methanol near about lambda max of 200 nm. so prefer to ACN

Is This Answer Correct ?    28 Yes 4 No

what is use of acetonitrile compare to methanol in RP-HPLC Method devlopment..

Answer / balaji

the uv cut oof value of methanol is 210 nm so we cant use
methanol near about lambda max of 200 nm. so prefer to ACN
& ACN is highly polar as compare to Methanol So provide
Better resolution for many compound and it has property to
form hydrogen bond so provide better selectivity.

Methanol viscosity more than the ACN. so ACN easly flow
through column

Is This Answer Correct ?    15 Yes 1 No

what is use of acetonitrile compare to methanol in RP-HPLC Method devlopment..

Answer / dharmesh radadiya

ACN is highly polar as compare to Methanol So provide Better
resolution for many compound and it has property to form
hydrogen bond so provide better selectivity.

Is This Answer Correct ?    17 Yes 10 No

what is use of acetonitrile compare to methanol in RP-HPLC Method devlopment..

Answer / bharat patel

same as answer 4

Is This Answer Correct ?    6 Yes 1 No

what is use of acetonitrile compare to methanol in RP-HPLC Method devlopment..

Answer / snehal

ACN is more polar and neutral solvent and has low viscosity than methanol hense shows low back pressure than methanol

Is This Answer Correct ?    7 Yes 3 No

what is use of acetonitrile compare to methanol in RP-HPLC Method devlopment..

Answer / guest

strength of elution varies among different organic solvents...among methanol and acetonitrile, acn has higher elution strength than methanol

Is This Answer Correct ?    1 Yes 3 No

what is use of acetonitrile compare to methanol in RP-HPLC Method devlopment..

Answer / rohithirpara

Dear Dharmesh
Methanol is very polar than ACN

Is This Answer Correct ?    7 Yes 28 No

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