what is use of acetonitrile compare to methanol in RP-HPLC
Method devlopment
Answers were Sorted based on User's Feedback
Answer / sandesh
the uv cut oof value of methanol is 210 nm so we cant use
methanol near about lambda max of 200 nm. so prefer to ACN
Is This Answer Correct ? | 28 Yes | 4 No |
Answer / balaji
the uv cut oof value of methanol is 210 nm so we cant use
methanol near about lambda max of 200 nm. so prefer to ACN
& ACN is highly polar as compare to Methanol So provide
Better resolution for many compound and it has property to
form hydrogen bond so provide better selectivity.
Methanol viscosity more than the ACN. so ACN easly flow
through column
Is This Answer Correct ? | 15 Yes | 1 No |
Answer / dharmesh radadiya
ACN is highly polar as compare to Methanol So provide Better
resolution for many compound and it has property to form
hydrogen bond so provide better selectivity.
Is This Answer Correct ? | 17 Yes | 10 No |
Answer / snehal
ACN is more polar and neutral solvent and has low viscosity than methanol hense shows low back pressure than methanol
Is This Answer Correct ? | 7 Yes | 3 No |
Answer / guest
strength of elution varies among different organic solvents...among methanol and acetonitrile, acn has higher elution strength than methanol
Is This Answer Correct ? | 1 Yes | 3 No |
Answer / rohithirpara
Dear Dharmesh
Methanol is very polar than ACN
Is This Answer Correct ? | 7 Yes | 28 No |
What is difference between the working standard and reference standard?
on saturation solubility study data how we can find out the bcs class of drug?
difference between precision & accuracy.
is there any exact definition for Theoritical plates ? chennu srinivas
what is hold time in Gc?
Why Deuterium lamp is used as UV light Source for UV.
What is the unit of Colour?
Difference between c18
6 Answers Apothecon, Dr Reddys, Intas, IPQA, SUN, Torrent Pharma,
what is mean by theoritical plates(not formula) ?
For standardization of volumetric solutions, acceptance limit for concentration is considered as 10% of nominal concentration and %RSD as NMT 0.2% (for appropriate no. of replicates). But , if %RSD has not met, what should be further action?
What is the difference between STANDARDIZATION and CALIBRATION of instrument like KF? why do we standardization of KF everyday? Why the KF factor being only 5.02 etc?
5 Answers AlpaMed, Vergo pharma research,
[3/30, 13:29] Manoj P Venkatpurwar: How hplc column selection according to structure? How mobile phase buffer selection on molecule structure?