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hii,
i'm had a sample of 1L that gone through extraction &
evaporation. i used 0.5ml of methanol for reconstitution
before injecting the sample to HPLC. i made a calibration
curve using a standard solution but now i'm confused about
how to display the concentration i got from the sample. for
ex. i got 0.2ug/ml how i'm gonna explane this is for the 1L
?
please help .. i'll really appreciate it
thanks
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Answer / ayyash
i think it reconstitution amount won't matter as long it's the same for all samples .. so the concentration of the sample should the represent the 1L !! so i think you can say the 0.2ug/ml is actually 0.2ug/L
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