Interested to Buy Any Domain ? << Click Here >> for more details...
How will you select mobile phase in HPLC Method Development
- 4 Answers
- 18081 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / maulik
based on the pka value of compound. if ur compound have pka
is about 3. u can select ur mobile phase about ph 5-14.but
due to the less column selectivity in basic media u can
select 5-8 pH. preferred by the different salt buffering
capacity.
| Is This Answer Correct ? | 19 Yes | 5 No |
Answer / rishabh
First of all check your compound solubility in which solvents then after that based on solubility the Mphase selection becomes easy if compound is soluble in aqueous medium check the pH of that medium than after further procced if not then check the solubility in organic solvents on basis of solubility and pH of the Mobile phase. Both Mobile phase selection and stationary phase are selected.
| Is This Answer Correct ? | 0 Yes | 0 No |
what is custom fields in waters system?
1 Answers Akorn India, Aurobindo,
UV and PDA detector, which have less signal to noise ratio?
what is peak purity? how may types of methods to determine peak purity?
what is the value of pH
How to choose the which salt is suitable for mobilephase
which light is used in FT-IR spectroscopy
why the ph scale range is 1 t0 14 and why not beyond 14 and not to below 1?
5 Answers Avra, Granules, Orchid,
why we wre doing linearity siudiec in hplc method validation
What is aggregate and fragments in SEC?
How to determine water content of thioanisole? which interfere by KF titration?
only one end point is possible for citric acid and we get 2 end points for di acid why?
Why we perform the ir analysis?
Organic Chemistry 
