How will you select mobile phase in HPLC Method Development
- 4 Answers
- 16873 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / maulik
based on the pka value of compound. if ur compound have pka
is about 3. u can select ur mobile phase about ph 5-14.but
due to the less column selectivity in basic media u can
select 5-8 pH. preferred by the different salt buffering
capacity.
| Is This Answer Correct ? | 19 Yes | 5 No |
Answer / rishabh
First of all check your compound solubility in which solvents then after that based on solubility the Mphase selection becomes easy if compound is soluble in aqueous medium check the pH of that medium than after further procced if not then check the solubility in organic solvents on basis of solubility and pH of the Mobile phase. Both Mobile phase selection and stationary phase are selected.
| Is This Answer Correct ? | 0 Yes | 0 No |
why base line not starts from zero in gc?
1 Answers Aurobindo, FDC, Reddy Labs,
What is polymorphism?which type of compounds exhibits polymorphism?
mixed 1.0 ml of methanol and 1.0 ml of toluene and injected in gas chromatography.will you prove that both solvents are in the ratio 1:1(not by area normalization)
How to Prepare Dilute Sodium hydroxide solution?
what is the principle of assay of sodium nitrite?
what is the relation of normality & molarity ? give me formula?
Please do not join if u got offer in oncotherapies ltd (STRIDESARCOLAB)especially Mr.Mahesh Bhatt team because job is not secure his team and doesn't have confidence on him becareful this offer?
Tell me something about Vitamin A test method by HPLC
What is the procedure for the calibration of ph meter?
why sucrose used in polarimeter calibration?
2 Answers Reddy Labs, Sugar Factory,
Can you please give a suitable definition and an example for understanding the words purity and potency.
6 Answers Cipla, Malladi Drugs and Pharmaceuticals, Reddy Labs,
WHICH PRESSURE IS HIGH EITHER INJECTOR OR DETECTOR IN GAS CHROMATOGRAPHY
4 Answers Avon Organics, Dr Reddys, Reddy Labs,
Organic Chemistry 
