hii,
i'm had a sample of 1L that gone through extraction &
evaporation. i used 0.5ml of methanol for reconstitution
before injecting the sample to HPLC. i made a calibration
curve using a standard solution but now i'm confused about
how to display the concentration i got from the sample. for
ex. i got 0.2ug/ml how i'm gonna explane this is for the 1L
?
please help .. i'll really appreciate it
thanks
Answer Posted / ayyash
i think it reconstitution amount won't matter as long it's the same for all samples .. so the concentration of the sample should the represent the 1L !! so i think you can say the 0.2ug/ml is actually 0.2ug/L
| Is This Answer Correct ? | 2 Yes | 3 No |
Post New Answer View All Answers
what is biorelivent dissolution media?
Related substance method equivalency on control sample or spiked sample?
Is it nessesary all multimedia dissolution require descriminatory?
for which product require to do content uniformity? what is limit of cu?
using gradient pressure in gas chromatography are not ?using gradient pressure why
in which situation require to take incident in validation?
before starting analytical method valodation what you checking? and how giving preference to start validation?
How we choose the mobile phase for method development.?
i want model written test paper for the post of analyst in IOCL(indian oil corporation ltd) any one pls help me
how to calibrate hplc & gc
what is classification of elemental impurities? what is risk assement in elemental impurities?
what is the extinction coefficient for Indomethacin or at 319nm wave length.
I have compare C2H2-air and C2H2-N2O flame AAS on determination calcium. Both use same range of std to plot calibration curve. (2-6ppm) When i measure the sample with phosphate, KCl and LaCl, C2H2-N2O flame give false positive result, around 0.5ppm. When i measure the sample with phosphste, KCl and EDTA. C2H2-N2O flame also give 0.5ppm false positive. But both above mentioned sample would not give false positive when measured by C2H2-air flame. What is the reason?
How to calibration of the uv spectroscopy and its test?
How to know the estimated LOQ concentration in ppm