What is the b/W dead volume and dwell volume ?
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Answer / falguni patel
Dead volume: It comprises the volume of HPLC system between
the point of injection to the point of detection, excluding
the part of column that contains packing.
Dwell volume: It includes the volume between pump and
column. in low pressure gradient it may be 1 mL & in high
pressure gradient it is to less.
| Is This Answer Correct ? | 14 Yes | 2 No |
Answer / aks
Dead Volume:Volume between injector to detector excluding
column volume (and also called as extra column volume)
Dwell Volume:Volume form the point of mixture to the column
entry (Also called as system delay volume)
| Is This Answer Correct ? | 10 Yes | 0 No |
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1 Answers Glochem, Sidmak Laboratories,
Organic Chemistry 
