why we use caffeine for hplc calibration?
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Answer / bhagavanji nelakanti
Caffeine is a primary standard which is having good
qualities like very soluble in methanol, highly stable at
room temperature and given set of conditions also. It is
having high boiling point, cheap in cost, easily available.
It is easy to prepare and handle and give good responses
(lambda max) at 205nm and 272 nm, (lambda Min) at 244 nm. We
can identify the difference in response with changing of
single nm also(271-273nm).
| Is This Answer Correct ? | 50 Yes | 0 No |
Answer / shivashankars,ils,hyd
Caffine is primary standard and Shows maxima at two
different wavelength ie.205nm & 273nm and Cheap & easily
available
| Is This Answer Correct ? | 10 Yes | 1 No |
Answer / david. r
Solutions of
potassium dichromate in perchloric acid and holmium oxide
in perchloric acid can be used.
However, these acidic solutions are difficult to work with
as the flow cell has to be thoroughly
cleaned after the measurement to remove any traces of
fluorescence from the potassium
dichromate solution. Aqueous caffeine solution, which is
easy to prepare and handle, with lmax at
272 nm and 205 nm, and lmin at 244 nm, can also be used.
| Is This Answer Correct ? | 8 Yes | 1 No |
Answer / jaimesh soni
caffeine having different wavelengths it having 2 maxima & 1 Minima . First maxima is 205 nm & second maxima is 273 nm . Minima is 245 nm. caffeine easily available in market. It is very stable we can use a long time for analysis (it is not degradable)
| Is This Answer Correct ? | 6 Yes | 0 No |
Answer / sarma
caffiene absorbence is the through out u.v range. so it can
be used.
| Is This Answer Correct ? | 1 Yes | 4 No |
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