Golgappa.net | Golgappa.org | BagIndia.net | BodyIndia.Com | CabIndia.net | CarsBikes.net | CarsBikes.org | CashIndia.net | ConsumerIndia.net | CookingIndia.net | DataIndia.net | DealIndia.net | EmailIndia.net | FirstTablet.com | FirstTourist.com | ForsaleIndia.net | IndiaBody.Com | IndiaCab.net | IndiaCash.net | IndiaModel.net | KidForum.net | OfficeIndia.net | PaysIndia.com | RestaurantIndia.net | RestaurantsIndia.net | SaleForum.net | SellForum.net | SoldIndia.com | StarIndia.net | TomatoCab.com | TomatoCabs.com | TownIndia.com
Interested to Buy Any Domain ? << Click Here >> for more details...

why we use caffeine for hplc calibration?

Answer Posted / david. r

Solutions of
potassium dichromate in perchloric acid and holmium oxide
in perchloric acid can be used.
However, these acidic solutions are difficult to work with
as the flow cell has to be thoroughly
cleaned after the measurement to remove any traces of
fluorescence from the potassium
dichromate solution. Aqueous caffeine solution, which is
easy to prepare and handle, with lmax at
272 nm and 205 nm, and lmin at 244 nm, can also be used.

Is This Answer Correct ?    8 Yes 1 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

What is related substance by HPLC impurity limits as per USP?

1153


why holium oxide prepared in 10% HCLO4 SOLUTION

1497


How to determine water content of bis tetrazole amine mono ammonium salt

3529


Please tell me about the pH of Polycaboxylic ether is it in the 5-6 range ever or more than 6

2652


If we do accuracy at same concentration at which linearity planned,what is the need to do linearity separately?

2347


what is the difference between potentiometric titration and karl fischer titration?

3257


mode of absorption in alimentary canal?

2434


if content uniformity passing but dissolution varrying then what is next step?

936


if rsd failed then what require to do?

879


for inorganic molecules require to do RS, Assay and disso?

774


if identification threshold crosses the limits then what next step?

915


Ratio of Water and Acetonitrile(diluent) is not recommended to use in cleaning method validation,why?

3024


For limit test of heavy metals in BP, Method C require that the substance is ignited at a temperature not exceeding 800 °C. Why confines such the temperature?

4134


why we are using benzene, anyline in acetic anhdride assay titration method?

2169


What is stray light in uv what are units for stray light

1720