Answer / kanakadurga

A solution of 0.005M H2SO4provides ionic strength to the
solution (hence, it changes the refractive index so as to be
similar to comparable solutions), but it doesn't absorb much
in the UV or Visible region. Therefore, light hitting the
detector will be almost all stray light.

Caffine is primary standard and Shows maxima at two
different wavelength ie.205nm & 273nm and Cheap & easily
available

Answer / sp goswami

A 0.005M sufuric acid solution gives the required ionic
strength, required dissolution and also do not interfere in
UV-VIS Region giving 99% absorbance for the analyte of
interest so 0.005 M diluted sulfuric acid is useful .
Caffeine is a primary std ,pure form ,good absorbance and
readily available in market
Regards

if you get peak in blank then what require to do?

0 Answers  

---

What is the exact reason to use THF and IPA in buffers of Reverse phase HPLC as a solvent?

1 Answers   Dr Reddys,

---

WHICH PRESSURE IS HIGH EITHER INJECTOR OR DETECTOR IN GAS CHROMATOGRAPHY

4 Answers   Avon Organics, Dr Reddys, Reddy Labs,

---

priciple of gc & hplc? (properly describe)

2 Answers   Cadila, Cipla, Reddy Labs,

---

how we do assay of urea, ammonium sulpate, ammonium nitro pospate etc

1 Answers   Alembic,

---

What is the diffrence between the performing of LOD ordinary drying at voven and vaccum method.

4 Answers   Apex,

---

What are the primary standards?

6 Answers  

---

why plane of polarized light rotated by opticaly active compunds?

1 Answers   Glenmark,

---

Basic principle of ESI?

0 Answers   Cipla, GVK,

---

why water, methanol, ethanol ets solvents are used in UV SPECTRIPHOTOMETER for blank reading ?

7 Answers  

---

What is linearity in HPLC and how will you test?

1 Answers  

---

What is FFA, PV, and PAV value in oil.

1 Answers   Nestle,

---