why 0.005 M sulphuric acid is used to calbrate
spectrophotometer and why caffiene is used for calibrating
hplc
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Answer Posted / sp goswami
A 0.005M sufuric acid solution gives the required ionic
strength, required dissolution and also do not interfere in
UV-VIS Region giving 99% absorbance for the analyte of
interest so 0.005 M diluted sulfuric acid is useful .
Caffeine is a primary std ,pure form ,good absorbance and
readily available in market
Regards
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