how calculate column dead volume in hplc ?
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Answer / subash
we know that K= tr-to/to......1
to=vm/F
where Vm=Volume of mobile phase inside the column
F= Flowrate
Vm= 0.5Xsuare of col diameter(cm)X column lenght(cm)
For a 150mm*4.6mm
Vm=0.5*0.2116*15
Vm=1.587 mL
| Is This Answer Correct ? | 10 Yes | 6 No |
Answer / jeegnesh zekadiya
Ï€r2l
3.14x(id/2)²xlenght
Exp= 0.150 Cm convert x(4.6/2)²x3.14
0.15x5.29x3.14= 2.49 Ded volume of coulnm
| Is This Answer Correct ? | 2 Yes | 4 No |
Answer / torrent
Dead volume (ml)= 0.387*2(Column Diameter)*Column Length
Column Dia.and Length in cm
| Is This Answer Correct ? | 5 Yes | 10 No |
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