Answer / subash

we know that K= tr-to/to......1

to=vm/F
where Vm=Volume of mobile phase inside the column
F= Flowrate

Vm= 0.5Xsuare of col diameter(cm)X column lenght(cm)
For a 150mm*4.6mm
Vm=0.5*0.2116*15
Vm=1.587 mL

Answer / jeegnesh zekadiya

Ï€r2l
3.14x(id/2)²xlenght
Exp= 0.150 Cm convert x(4.6/2)²x3.14
0.15x5.29x3.14= 2.49 Ded volume of coulnm

Answer / torrent

Dead volume (ml)= 0.387*2(Column Diameter)*Column Length
Column Dia.and Length in cm

Optical Rotation*0.9477*100*200] Assay Of dextrose= ________________________________ 99.8* 200 why Factor=0.9477

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