Answer Posted / subash

we know that K= tr-to/to......1

to=vm/F
where Vm=Volume of mobile phase inside the column
F= Flowrate

Vm= 0.5Xsuare of col diameter(cm)X column lenght(cm)
For a 150mm*4.6mm
Vm=0.5*0.2116*15
Vm=1.587 mL

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