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What is the difference between Normal phase method &
Reverse method in HPLC ? Define application of both method.

Answers were Sorted based on User's Feedback



What is the difference between Normal phase method & Reverse method in HPLC ? Define applicati..

Answer / k l srinivas

Normal phase: Stationary phase is polar, mobile phase is non
polar,
Reverse phase: Stationary phase is non polar, mobile phase is
polar.

Is This Answer Correct ?    57 Yes 1 No

What is the difference between Normal phase method & Reverse method in HPLC ? Define applicati..

Answer / maulik

Normal phase: Stationary phase is polar, mobile phase is non
polar,
Reverse phase: Stationary phase is non polar, mobile phase is
polar.

normal phase is based on the adsorption phenomenon and
reverse phase is based on the partition phenomenon

normal phase used for chiral compound and reverse phase used
for other

Is This Answer Correct ?    20 Yes 2 No

What is the difference between Normal phase method & Reverse method in HPLC ? Define applicati..

Answer / srikanth

above answer is perfect and also,,,....

Normal Phase: separates analytes on the basis of their
affinity for a polar stationary surface such as silica.

Reversed Phase: separates on the basis hydrophobic
interacertions, which originates from the high symmetry in
the dipolar water structure

Is This Answer Correct ?    7 Yes 0 No

What is the difference between Normal phase method & Reverse method in HPLC ? Define applicati..

Answer / ram krishna

Normal phase _Column is polar and more is non polar sudh as IPA hexane
Reversed phase_Column is non polar and more is polar such as water and acetonitrile mp

Is This Answer Correct ?    2 Yes 0 No

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