What is the difference between Normal phase method &
Reverse method in HPLC ? Define application of both method.
- 4 Answers
- 19632 Views
- I also Faced
- E-Mail Answers
Answer Posted / srikanth
above answer is perfect and also,,,....
Normal Phase: separates analytes on the basis of their
affinity for a polar stationary surface such as silica.
Reversed Phase: separates on the basis hydrophobic
interacertions, which originates from the high symmetry in
the dipolar water structure
| Is This Answer Correct ? | 7 Yes | 0 No |
Post New Answer View All Answers
How do we fix the sample concentaryion in hplc method development?
what is biorelivent dissolution media?
why we are using hexane in calibration of number of drop per mL
For limit test of heavy metals in BP, Method C require that the substance is ignited at a temperature not exceeding 800 °C. Why confines such the temperature?
in sampling of raw material which side is pasted sampled labels
on the basis of bcs class how to proceed the solubility?
how the compound separate in coulunm,explain
which one is better separation in high pressure liquide chromatogram orlowpressure chromato gram in hplc
for heavy metal test lead used in which form pure form or any other form for preparation of lead standard sol
why require to add enzymes in capsules dissolution only? is it require to tablet also?
Why only hydroxy naphthol blue indicator is used for standardisation of 0.05M EDTA solution instead of solochrome Black T or Euriochrome Black T indicator which is used for all sample analysis with 0.05M EDTA solution?
How can we calculate "confidence interval" in analytical method validation? Pl. explain with example.
How much is the standard area for glc analysis
in which situation ion pair require to use?
How to determine water content of bis tetrazole amine mono ammonium salt
