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What is the difference between Normal phase method &
Reverse method in HPLC ? Define application of both method.
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Answer Posted / maulik
Normal phase: Stationary phase is polar, mobile phase is non
polar,
Reverse phase: Stationary phase is non polar, mobile phase is
polar.
normal phase is based on the adsorption phenomenon and
reverse phase is based on the partition phenomenon
normal phase used for chiral compound and reverse phase used
for other
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