Interested to Buy Any Domain ? << Click Here >> for more details...
what buffer you can select for acidic nature analyte and
how ?
- 2 Answers
- 13753 Views
- Dr Reddys, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / shyam kumar
For an acidic nature compound u should use only acid pH
buffers only why because acid compounds are showing more
hydrofobicity in acid pH, if the sample is having more
hydrofobicity it retains on column and it gives good peak
shape and advisable retention time,
if we do acid samples in basic pH buffers the sample
undergoes ionisation meanwhile increases the hydrophilic
nature dueto this hydrophilic nature the sample not retains
on the column. u must select a acid pH to analyse the acid
samples.
| Is This Answer Correct ? | 38 Yes | 0 No |
Answer / m
Selection of buffer pH is depend on the pKa Value of
compound as you say the nature of compound is acidic so i
will prefer the acidic buffer near pH about +-1 of pKa value
of analyte and the buffer with acidic pH range i prefer
phospahte buffer because it is depend the buffer pKa also.
| Is This Answer Correct ? | 7 Yes | 3 No |
how can give the expiry period and restadardisation of volumetric solution
0 Answers Meenaxy Pharma, SRF,
Why we use 100 RPM in friability test
what is the difference between c-8 and c-18 column
pda detector mechanism?
1 Answers DKR, Teva Pharmaceuticals,
How can we konw that the HPLC column is endcapped? Is it possible to identify visually without COA?
Why chromic acid wash is not recommended by USP for glassware cleaning?
before starting analytical method valodation what you checking? and how giving preference to start validation?
How they found 1mL of K.F reagent is equivalent to 5mg of water and if we change the composition of K.F reagent, is it can neutralize more amount of water?
In the gas chromatography what is used carrier gas and make up gas?
How we will choose pH of a mobile phase if analyte pKa value is 4
What is basic difference between method validation,process validation
In case of HPLC coloums what is the difference Between C8 & C18 coloums & how can we select difference type of coloums like Inertial ODS , Hypersil BDS , ODS etc.?
Organic Chemistry 
