what buffer you can select for acidic nature analyte and
how ?
Answer Posted / shyam kumar
For an acidic nature compound u should use only acid pH
buffers only why because acid compounds are showing more
hydrofobicity in acid pH, if the sample is having more
hydrofobicity it retains on column and it gives good peak
shape and advisable retention time,
if we do acid samples in basic pH buffers the sample
undergoes ionisation meanwhile increases the hydrophilic
nature dueto this hydrophilic nature the sample not retains
on the column. u must select a acid pH to analyse the acid
samples.
Is This Answer Correct ? | 38 Yes | 0 No |
Post New Answer View All Answers
if rsd failed then what require to do?
how the compound separate in coulunm,explain
what is mean by extactable and leachable study?
what is mean by covalidation
for heavy metal test lead used in which form pure form or any other form for preparation of lead standard sol
what is mean by 40 in the dissolution basket mesh size?
in OSD forms require to use gas chromatography?
what are the standard methods for HPLC?
What is diffrence between extractable volume and deliverable volume? Answer pls
How will u perform degradation study in detectors other thann PDA in HPLC Methodology
is it nessesary to do solution stability for 7 days?
[3/30, 13:29] Manoj P Venkatpurwar: How hplc column selection according to structure? How mobile phase buffer selection on molecule structure?
if you get peak in blank then what require to do?
what you do when compound having dwel wavelength?
What is intact assay method development.....?