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Why we use Uracil for HPLC calibration in Detector wave
ength accuracy test?
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Answer Posted / gangi reddy
Since it is a standard, Its a unretained substance in the
HPLC Column. No column is required for its elution.
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2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.
