Answer / bob

At 664, it's 74028. Extinction coefficient is not dependent
on concentration, but absorption is directly dependent on
concentration and the distance (length) that the light must
travel through the solution. So you can use a very thin
layer of concentrated solution, or a test-tube full of a
less-concentrated solution to get the same absorbance.

A = e*c*d.
e = extinction coefficent
c = concentration (molarity)
d = depth (optical path length)

Answer / alias

hi, u can gt the absorbancies from ths reely good table
here.. http://omlc.ogi.edu/spectra/mb/mb-water.html

thanks.

:)

Answer / girish padhy

I have no idea of the value of the molar extinction
coefficient of methylene blue at wavelenght 664 nm but for
the second question, I have a dual answer.First answer
is "NO DEPANDANCE" for dilute solutions i.e.,the
concentration upto which the molecules in the solution
which do not form any di, tri or polymers.But if the
solution is made more concentrated then there is a tendency
for the molecules to form polymers.These polymers have a
tendency to absorb with wifferent absorption coefficient
then their corresponding monomers.

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