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How much is the molar extinction coefficient of methylene
blue at wavelenght 664 nm? Are molar extinction coefficient
depend on concentration of solution?
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Answer Posted / bob
At 664, it's 74028. Extinction coefficient is not dependent
on concentration, but absorption is directly dependent on
concentration and the distance (length) that the light must
travel through the solution. So you can use a very thin
layer of concentrated solution, or a test-tube full of a
less-concentrated solution to get the same absorbance.
A = e*c*d.
e = extinction coefficent
c = concentration (molarity)
d = depth (optical path length)
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