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What is the diffrence in japan mkt requirement in analytical method validation over US?


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Many times I don't got a caffeine peak in calibration of hplc using guard column ❓

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what is lod and loq ?,why use k2cr2o7 , kcl h2so4 in uv calibration ?,why use benzophenone & caffene acetone in hplc calibration ?,what is leading peak in hplc ?why we do the calibration of limit of stry light in hplc & uv ?

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why we are performing residue on ignition?

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ash or residue in that what will be there.

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In HPLC calibration, caffeine is used as primary standard for wave length calibration due to caffeine is having dual maxima at 273 & 205 nm and one minima at 245 nm. Any body can give reference of these details from any pharmacopeia (with chapter no.) or any other authentic guideline?

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what is diffrence between specificity and selecivity?

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In UV we measure absorbance , how it is happend ? explain me

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