Question 89 - A hemocytometer is a device that is used for counting cells. In an engineering experiment, 100 microlitres of cell suspension is diluted with 50 microlitres of Trypan blue dye. Only death cells appear blue in color when stained with the dye. There are 57 cells detected in a hemocytometer, where 5.3 % of them appear blue when the chamber of the meter is placed under a microscope. Each square of a chamber can contain 0.0001 mL of liquid. (a) Calculate the number of viable cells. (b) The cells ocupied 5 squares. Calculate the average number of viable cells / square. (c) Calculate the dilution factor of the cell suspension by using the formula : Dilution = final volume / initial volume. (d) Calculate the concentration of viable cells / mL by using the formula : Concentration = (Average number of viable cells / square) x dilution x (square / volume).

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What are the uses of quicklime?

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What parameters should be considered while selecting type of filter?

4 Answers   Nocil,

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how much inducation voltage shows in 11 kv

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In differential centrifugation of cells with diameter D in centimeter, the square of D is given by D x D = [18n ln (RF / RI) ] / [ (RP – RFF) Wt ] where n is the fluid viscosity (poise), RF is the final radius of rotation (cm), RI is the initial radius of rotation (cm), RP is cell density (g/ml), RFF is the fluid density (g/ml), W the square for the rotational velocity in (radians / s) (radians / s), t is the time required to sediment from RI to RF (s). Derive an equation for W as a function for D, n, RF, RI, RP, RFF and t, with the stated units above, in radian

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How can separation of chiral chemicals affect the chemical and/or pharmaceutical industries?

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What are the apt definitions for apparent power, active power and reactive power?

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What is the procedure to estimate the friction factor involved in heat exchanger tubes?

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Is there a quick rule-of-thumb to estimate a gas side heat-transfer rate inside the tubes of a shell and tube heat exchanger?

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In Galvonised iron and stainsteel pipes, in which pipe friction losses will more and why ?

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Question 64 – According to a heuristic of chemical engineering plant design, assume a pressure difference dP = 4 psi for each 10-ft rise in elevation. A pump is needed to pump liquid from a storage tank at a lower elevation to a heating tank at a higher elevation with the elevation difference of 60 ft. (a) Find the pressure loss due to such elevation. (b) If the required minimum inlet pressure to the heating tank is 9 psi, with 1 control valve is installed between pump and heating tank, what is the dP minimum required for the control valve and the entrance to the heating tank when the heuristic mentions that at least 10 psi is required for the control valve? (c) The pressure at the inlet of the pump is 8 psi and the flowrate of the liquid produces pressure head of 50 psi. What is the total pressure produced by the pump? (d) Assume a pipeline dP of 2 psi / 100 ft for liquid flow in a pipe according to heuristic, what is the approximate maximum length of the pipe in ft that can be installed between the pump and the heating tank?

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What is meant by surging in compressors?

5 Answers   CPCL,

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