BIOCHEMICAL ENGINEERING INSTRUMENTATION - EXAMPLE 29.1 : In the application of T

BIOCHEMICAL ENGINEERING INSTRUMENTATION - EXAMPLE 29.1 : In the application of Theory of Spectrometry in spectrophotometer, let n = N x C x V, V = A x t, e = a x N where n = number of molecules, N = Avogadro's number, V = volume of cuvette, A = area of cuvette, t = thickness of cuvette, C = concentration of fluid in the cuvette, e = extinction coefficient, a = effective area of molecule. (a) By using calculus in dI = -I x a x N x C x dt, prove that ln (I / Io) = -a x N x C x t, where dI is the small difference in I and dt is the small difference in t. I = intensity of light. Io = initial intensity of light. (b) Show by calculations that ln (Io / I) = e x C x t based on the answer in the previous question (a). (c) Find the equation of log (Io / I) as a function of e, C and t based on the answer in the previous question (b).

Question Posted / kangchuentat

1 Answers
2127 Views
I also Faced
E-Mail Answers

BIOCHEMICAL ENGINEERING INSTRUMENTATION - EXAMPLE 29.1 : In the application of Theory of Spectrometr..

Answer / kangchuentat

BIOCHEMICAL ENGINEERING INSTRUMENTATION - ANSWER 29.1 : (a) Let dI = -I x a x N x C x dt, then dI / I = -a x N x C x dt. Integrate both sides of the equation gives ln (I / Io) = -a x N x C x t. (b) When ln (I / Io) = -a x N x C x t and e = a x N, then ln (I / Io) = -e x C x t. If ln (I / Io) = - ln (Io / I), then ln (Io / I) = e x C x t. (c) Let ln (Io / I) = log (Io / I) / log E, where E = 2.718 approximately. Then log (Io / I) = log E x ln (Io / I) = log E x e x C x t. The answer is given by Kang Chuen Tat; PO Box 6263, Dandenong, Victoria VIC 3175, Australia; SMS +61405421706; chuentat@hotmail.com; http://kangchuentat.wordpress.com.

Is This Answer Correct ?

0 Yes

0 No

Post New Answer

More Chemical Engineering Interview Questions

what is zeroth law ?

6 Answers ISRO,

What's the difference between superphosphate and triple super phosphate fertilizer?

4 Answers

how many TDS & TOTAL HARDNESS, PH of drinking water is recomanded for human being use.

4 Answers

Explain what is the significance of the minimum flow required by a pump?

0 Answers

Explain what type of pump may be appropriate for a liquid near saturation, a low flow rate, and very limited npsha (net positive suction head available)?

0 Answers

What factors go into designing the vapor space of kettle type reboiler?

0 Answers

HOw to derive reynolds equation?

1 Answers

What is the purpose of capacitor?

0 Answers

I want to need previous placement questions of Jindal

1 Answers Jindal, Jindal Steel and Power,

What does the catalytic converter on an automobile do?

0 Answers

What is some common piping materials used to transport slurries?

0 Answers

Question 87 - According to Hardy-Weinberg Equation, p x p + 2 x p x q + q x q = 1 where p = dominant allele frequency and q = recessive allele frequency. Let p + q = 1. Fraction of population has 2 copies of the p gene = p x p. Fraction of population has 2 copies of the q gene = q x q. Fraction of population has a copy of p gene and a copy of q gene = 2 x p x q. In a small town, the allele frequency is q = 0.2 for a recessive gene, the delta-32 mutation, that gives human protection from HIV infection. (a) Find the allele frequency a dominant gene, p. (b) What percent of the population has at least a copy of the gene that cause the population either immune to HIV or less susceptible to the disease?

1 Answers

For more Chemical Engineering Interview Questions Click Here