what is the difference between hplc and uplc?
Answers were Sorted based on User's Feedback
Answer / rajasekhar
In UPLC Components Seperated fastly than HPLC.so Run time very short in UPLC
Is This Answer Correct ? | 4 Yes | 0 No |
Answer / chandra sekhar reddy
in hplc particle size 2.2-5 micrometers and in uplc size 2 r below 2
Is This Answer Correct ? | 1 Yes | 1 No |
Answer / firoz
In hplc column partical size 2.5 to 5um or in uplc column partical size 1.7um.
Hplc run time higher than uplc
Solvent most use in hplc
Pressure lower than uplc upto 15000 to 18000
Is This Answer Correct ? | 0 Yes | 0 No |
Answer / brahmanandareddy
difference between the hplc and uplc only capacity of the pressure limits, hplc pressure limits up to 5000 psi-6000 psi, uplc pressure limits up to 1800 psi
Is This Answer Correct ? | 3 Yes | 15 No |
how to prepare working standard in our laboratory aganist reference standard ?
4 Answers Merit Biolabs, Unichem,
effect of pore size, pore volume, partical size, column length, carbon load on retention time? what is carbon load? what is the use?
How can we konw that the HPLC column is endcapped? Is it possible to identify visually without COA?
In kf constant term 16 what it is explain ?
I got 88.0 % assay for a drug in HPLC analysis for same injection i got 40.0% related substances is it possible? what is the relation between results of assay and purity?
in api coa contains only process impurities and in product coa contains degradation impurities?
What's the importance of these procedures?
what is the method of UV cabinet CALIBRTION
what is mean by ambient temperature?
in which situation ion pair require to use?
In lcms analysis some times we get sodium and potassium adducts. if the compound is havine sodium ions how can we confirm wether sodium is from sample or it is a adduct?
2 Answers Teva Pharmaceuticals,
class of solvent regarding Residual solvent e.g. Acetone his class