In GC, What does 'Column condensation test' mean by?
- 2 Answers
- 10198 Views
- Reddy Labs, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / sri krishna
Condensation Test
Use this test whenever injector or carrier gas contamination
problems are suspected (e.g., ghost peaks or erratic baselines).
* Leave the GC at 40-50�C for 8 or more hours.
* Run a blank analysis (i.e., start the GC, but with no
injection) using the normal temperature conditions and
instrument settings.
* Collect the chromatogram for this blank run.
* Immediately repeat the blank run as soon as the first
one is completed. Do not allow more than 5 minutes to elapse
before starting the second blank run.
* Collect the chromatogram for the second blank run and
compare it to the first chromatogram.
* If the FIRST chromatogram contains a substantially
larger amount of peaks and baseline instability, then that
is an indication that there is contamination upstream of the
capillary column (ie. contaminated inlet, dirty carrier gas,
etc.).
* If BOTH chromatograms contain few peaks or very little
baseline drift, it can be assumed that the carrier gas
and/or inlet are relatively clean.
* If BOTH chromatograms contain a significant amount of
noise and/or baseline drift, then that usually is an
indication that the detector or detector gases are contaminated.
| Is This Answer Correct ? | 8 Yes | 1 No |
Answer / guest
retention time per puss we r using mobile phase ph
| Is This Answer Correct ? | 0 Yes | 0 No |
what is karl fischer principle and reaction ,describe it
5 Answers Jubilant Organosys, Microsoft,
why PH range 0-14 explain
5 Answers DRL, Reddy Labs, Saint Gobain, Viva,
What is the procedure for UV-spectrophotometer calibration?
How you develop a method in HPLC?
0 Answers CU, Perrigo, Wockhardt,
on what basis SOP's can be changed? IF mobile phase ratio is 70:30 to how much % can we change the ratio in HPLC process
signifcance of detector lenearity in calibration f hplc
why kf factor coming 0.5
what is the moisture content of Disodium hydrogen tartrate. how it can be calculted. as 16.5%
In the HPLC Calibration done wavelength accuracy done between 200nm-280nm .but not done remaining 300-400nm not done ?
if v use HELIUM & ARGON instead of NITROGEN as a mobile phase what happen in GC SYSTEM ?
Why forced degradation studies are performed in hplc method validation?
Why will a solution of aspirin without acid or basic hydrolysis give yellow colour with a solution of Ferric chloride?
Organic Chemistry 
