Why using 0.5% acetone in methanol mobile phase for HPLC
Gradient performance check.

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Why using 0.5% acetone in methanol mobile phase for HPLC Gradient performance check...

Answer / babu1029

Acetone wavelength maxima is 260nm and it is freely soluble in water or methanol

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Why using 0.5% acetone in methanol mobile phase for HPLC Gradient performance check...

Answer / panchanan basantia

0.5% acetone in methanol shows uv cutoff.

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