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Why Urasil and Caffeine use for HPLC wavelength accuracy
calibration. which is most suitable, Why? other than these
which can be used?
- 7 Answers
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Answer / hemadri
Because it is stable in any condition . Easily availability in market. It will give sharp peak and run time also less.
| Is This Answer Correct ? | 13 Yes | 3 No |
Answer / suresh polisetti
bcoz these r gives the both abs max & min in the UV Region which means max= 205,273,min=245,
| Is This Answer Correct ? | 10 Yes | 0 No |
Answer / manish
caffeine and urasil solution is more stable at rt and show
multiweve length .
| Is This Answer Correct ? | 4 Yes | 1 No |
Answer / rishabh
anthracene and holomium oxide can also used for wavelength accuracy detector
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / mahendra
Because it give dual wavelength absorbance like 245nm 350 nm
| Is This Answer Correct ? | 5 Yes | 12 No |
Answer / chemistry
Because uracil And Caffierne Shows The absorbance Range in
245nm And 340nm.
| Is This Answer Correct ? | 0 Yes | 7 No |
Answer / gaurav oza
caffeine and urasil does not retain in HPLC. They are
generally used for measuring the dead volume or void volume
, so both are used for calibration of HPLC column
| Is This Answer Correct ? | 2 Yes | 10 No |
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We use Potassium Dichromate solution for the calibration of UV-Visible Spectrophotometer in UV region. My question is to calibrate visible region which solution could be used in photometric accuracy
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