Why Urasil and Caffeine use for HPLC wavelength accuracy
calibration. which is most suitable, Why? other than these
which can be used?
Answer Posted / mahendra
Because it give dual wavelength absorbance like 245nm 350 nm
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if your impurity coeluting with each other in that situation how require to set specification? is it acceptable?
In HPLC calibration, caffeine is used as primary standard for wave length calibration due to caffeine is having dual maxima at 273 & 205 nm and one minima at 245 nm. Any body can give reference of these details from any pharmacopeia (with chapter no.) or any other authentic guideline?
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