Why noise test is performed at 254 nm in calibration of HPLC
system?
Answers were Sorted based on User's Feedback
Answer / paresh jobanputtra, piramal
Earlier fixed wavelength detector is mostly used i.e 254 nm
because almost all Benzene ring containing compounds absorbs uv light
at 254 nm. i think due to this reasons only.
Regards,
Paresh Jobanputtra
Is This Answer Correct ? | 18 Yes | 1 No |
Answer / tejas shah
At 254nm none of the solvent or its impurities show significant interference so the noise will be recorded of pure UV light. i.e. detector noise
Is This Answer Correct ? | 0 Yes | 0 No |
Answer / jayesh patel
Generally all organic compounds have absorption at this
wavwlength.
Is This Answer Correct ? | 3 Yes | 6 No |
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