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Why noise test is performed at 254 nm in calibration of HPLC
system?
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Answer / paresh jobanputtra, piramal
Earlier fixed wavelength detector is mostly used i.e 254 nm
because almost all Benzene ring containing compounds absorbs uv light
at 254 nm. i think due to this reasons only.
Regards,
Paresh Jobanputtra
| Is This Answer Correct ? | 18 Yes | 1 No |
Answer / tejas shah
At 254nm none of the solvent or its impurities show significant interference so the noise will be recorded of pure UV light. i.e. detector noise
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / jayesh patel
Generally all organic compounds have absorption at this
wavwlength.
| Is This Answer Correct ? | 3 Yes | 6 No |
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