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Why noise test is performed at 254 nm in calibration of HPLC
system?

Answers were Sorted based on User's Feedback



Why noise test is performed at 254 nm in calibration of HPLC system?..

Answer / paresh jobanputtra, piramal

Earlier fixed wavelength detector is mostly used i.e 254 nm
because almost all Benzene ring containing compounds absorbs uv light
at 254 nm. i think due to this reasons only.

Regards,
Paresh Jobanputtra

Is This Answer Correct ?    18 Yes 1 No

Why noise test is performed at 254 nm in calibration of HPLC system?..

Answer / tejas shah

At 254nm none of the solvent or its impurities show significant interference so the noise will be recorded of pure UV light. i.e. detector noise

Is This Answer Correct ?    0 Yes 0 No

Why noise test is performed at 254 nm in calibration of HPLC system?..

Answer / jayesh patel

Generally all organic compounds have absorption at this
wavwlength.

Is This Answer Correct ?    3 Yes 6 No

Why noise test is performed at 254 nm in calibration of HPLC system?..

Answer / dhanji15

high wavelength

Is This Answer Correct ?    7 Yes 17 No

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