In GC, What does 'Column condensation test' mean by?
- 2 Answers
- 10215 Views
- Reddy Labs, I also Faced
- E-Mail Answers
Answer Posted / sri krishna
Condensation Test
Use this test whenever injector or carrier gas contamination
problems are suspected (e.g., ghost peaks or erratic baselines).
* Leave the GC at 40-50�C for 8 or more hours.
* Run a blank analysis (i.e., start the GC, but with no
injection) using the normal temperature conditions and
instrument settings.
* Collect the chromatogram for this blank run.
* Immediately repeat the blank run as soon as the first
one is completed. Do not allow more than 5 minutes to elapse
before starting the second blank run.
* Collect the chromatogram for the second blank run and
compare it to the first chromatogram.
* If the FIRST chromatogram contains a substantially
larger amount of peaks and baseline instability, then that
is an indication that there is contamination upstream of the
capillary column (ie. contaminated inlet, dirty carrier gas,
etc.).
* If BOTH chromatograms contain few peaks or very little
baseline drift, it can be assumed that the carrier gas
and/or inlet are relatively clean.
* If BOTH chromatograms contain a significant amount of
noise and/or baseline drift, then that usually is an
indication that the detector or detector gases are contaminated.
| Is This Answer Correct ? | 8 Yes | 1 No |
Post New Answer View All Answers
What are usp limits for theoritical plats,resoution,tailing factor,peak to valley ratio
In which situation we require to prepare the standard solution from sample in Related substance method?
how many types of balance calibration test?
AT WHAT CONDITIONS WILL YOU ANALYSE A SAMPLE,WHICH ARE PRESERVED AT OTHER THAN THE AMBIENT CONDITIONS?(ie COLD STORAGE SAMPLES BELOW 20* CENTIGRADE?
What is the difference between purge septum flow and column flow in gas chromatography?
why should we perform dissolution PVT calibration only by UV spectrometer, not by HPLC ?
What is viod volume and peak purity in HPLC?
why we should take dst factor for below 1%moisture samples
what is partion and column chromatography
How to calculate coreletion coefficient
How can we calculate "confidence interval" in analytical method validation? Pl. explain with example.
Why we used in n-butyl acetate water content terminology while in ethyl acetate we used moisture content terminology?
why are measure gas flow " ml " in Gas chromatography
How to calibration of the uv spectroscopy and its test?
what is mean by covalidation
