In GC, What does 'Column condensation test' mean by?
- 2 Answers
- 10473 Views
- Reddy Labs, I also Faced
- E-Mail Answers
Answer Posted / sri krishna
Condensation Test
Use this test whenever injector or carrier gas contamination
problems are suspected (e.g., ghost peaks or erratic baselines).
* Leave the GC at 40-50�C for 8 or more hours.
* Run a blank analysis (i.e., start the GC, but with no
injection) using the normal temperature conditions and
instrument settings.
* Collect the chromatogram for this blank run.
* Immediately repeat the blank run as soon as the first
one is completed. Do not allow more than 5 minutes to elapse
before starting the second blank run.
* Collect the chromatogram for the second blank run and
compare it to the first chromatogram.
* If the FIRST chromatogram contains a substantially
larger amount of peaks and baseline instability, then that
is an indication that there is contamination upstream of the
capillary column (ie. contaminated inlet, dirty carrier gas,
etc.).
* If BOTH chromatograms contain few peaks or very little
baseline drift, it can be assumed that the carrier gas
and/or inlet are relatively clean.
* If BOTH chromatograms contain a significant amount of
noise and/or baseline drift, then that usually is an
indication that the detector or detector gases are contaminated.
| Is This Answer Correct ? | 8 Yes | 1 No |
Post New Answer View All Answers
in each media we require to use SLS? how to proceed?
if rsd failed then what require to do?
in which situation require to change rs specification?
How to choose the which salt is suitable for mobilephase
can we use the same detector in HPLC as well GC and what could be the differences we can find in the final chromato graph in any aspects?
what is mean by dissolution biowaiver study?
acceptance criteria for lod & loq by standard deviation of response and slope??
In which situation we require to analytical method validation of excipient?
how to calculate elemental impurities?
What is the difference between chromatographic purity and related substances?
function of detecter in hplc ,gc and spectroscopy? function of carrier gas in gc?
in which situation ion pair require to use?
How doing qbd practically?
Why are use silicon oil mr?
what is partion and column chromatography
