what is the significant of pH in HPlc?
Answers were Sorted based on User's Feedback
selecting the pH of M.P. is important to get credible
retention time and is based on the pKa value, polar or
non-polar nature of the analyte.
| Is This Answer Correct ? | 33 Yes | 1 No |
Answer / roshan bhaskar
pH can change the hydrophobic character of the analyte. For this reason most methods use a buffering agent, such as sodium phosphate, to control the pH. Buffers serve multiple purposes: control of pH, neutralize the charge on the silica surface of the stationary phase and act as ion pairing agents to neutralize analyte charge.
| Is This Answer Correct ? | 2 Yes | 1 No |
Answer / pawan gupta
pH is the main role in M.P. pH depend retention time &peaks Sharp
| Is This Answer Correct ? | 0 Yes | 0 No |
what is the peak purity concept?
5 Answers Graviti Pharmaceuticals, Vimta Labs,
What is the difference between c8 and c18 hplc column?
why hexane is used for FID calibration in GC
what is icdas abbrivation
What is the difference between Optical rotation and Spcefic optical rotation
differnc btw c18 and c-8 column
In GC, What does 'Column condensation test' mean by?
What is Fourier Transform Spectrosopy?
5 Answers Aurobindo, Hetero, Lupin,
What is differences between the Instrument and Equipment
how can standardized of HCl of 0.1 N
full name of OOS & OOT ? Dercribe it with details ? why this is needed in pharma industry ?
why sucrose not used in polarimeter calibration?