what is the significant of pH in HPlc?
Answers were Sorted based on User's Feedback
selecting the pH of M.P. is important to get credible
retention time and is based on the pKa value, polar or
non-polar nature of the analyte.
| Is This Answer Correct ? | 33 Yes | 1 No |
Answer / roshan bhaskar
pH can change the hydrophobic character of the analyte. For this reason most methods use a buffering agent, such as sodium phosphate, to control the pH. Buffers serve multiple purposes: control of pH, neutralize the charge on the silica surface of the stationary phase and act as ion pairing agents to neutralize analyte charge.
| Is This Answer Correct ? | 2 Yes | 1 No |
Answer / pawan gupta
pH is the main role in M.P. pH depend retention time &peaks Sharp
| Is This Answer Correct ? | 0 Yes | 0 No |
What is Standard deviation
what is mean by Q in S1 and S2
why peak purity not considered in GC?
4 Answers DRL, Reddy Labs, Vimta Labs,
wt is difference between assay and purity in hplc?
Why we show ammonia vapours to tlc plate?to which compounds?
what is Fourier Transform
3 Answers Graviti Pharmaceuticals, GVK,
signifcance of detector lenearity in calibration f hplc
why we make titiration of Iron with EDTA ?
How to fix the concentration for RS by HPLC
Column selection in HPLC method development?
How can we confirm the HPLC column is end-capped or not? Is it possible to identify by physical appearance?
How are PDA Detector work in HPLC