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what is the significant of pH in HPlc?

Answers were Sorted based on User's Feedback



what is the significant of pH in HPlc?..

Answer / subramanya

selecting the pH of M.P. is important to get credible
retention time and is based on the pKa value, polar or
non-polar nature of the analyte.

Is This Answer Correct ?    33 Yes 1 No

what is the significant of pH in HPlc?..

Answer / karunasri

2-7.5

Is This Answer Correct ?    8 Yes 7 No

what is the significant of pH in HPlc?..

Answer / roshan bhaskar

pH can change the hydrophobic character of the analyte. For this reason most methods use a buffering agent, such as sodium phosphate, to control the pH. Buffers serve multiple purposes: control of pH, neutralize the charge on the silica surface of the stationary phase and act as ion pairing agents to neutralize analyte charge.

Is This Answer Correct ?    2 Yes 1 No

what is the significant of pH in HPlc?..

Answer / pawan gupta

pH is the main role in M.P. pH depend retention time &peaks Sharp

Is This Answer Correct ?    0 Yes 0 No

what is the significant of pH in HPlc?..

Answer / tushar

4-7.5pH

Is This Answer Correct ?    16 Yes 17 No

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