what is the significant of pH in HPlc?
Answers were Sorted based on User's Feedback
selecting the pH of M.P. is important to get credible
retention time and is based on the pKa value, polar or
non-polar nature of the analyte.
Is This Answer Correct ? | 33 Yes | 1 No |
Answer / roshan bhaskar
pH can change the hydrophobic character of the analyte. For this reason most methods use a buffering agent, such as sodium phosphate, to control the pH. Buffers serve multiple purposes: control of pH, neutralize the charge on the silica surface of the stationary phase and act as ion pairing agents to neutralize analyte charge.
Is This Answer Correct ? | 2 Yes | 1 No |
Answer / pawan gupta
pH is the main role in M.P. pH depend retention time &peaks Sharp
Is This Answer Correct ? | 0 Yes | 0 No |
why octanol used to determine the partition coefficient ?
Why we are using only KBr in IR spectrometer why not KCl
how to Calibrate FID IN GC
Why dissolution test apparatus calibration with salycilic acid tablets was stopped by FDA&USP from the year of 2009?
From peak purity spectrum, where or at which point peak purity angle & purity threshold is checked?
what is assay in hplc
what is optical active and non active give examples?
What is Impuriteis?
if you have given one product then how you determine the impurity in that?
how can give the expiry period and restadardisation of volumetric solution
0 Answers Meenaxy Pharma, SRF,
In which situation we require to analytical method validation of excipient?
%lod always grater than %water content for same product??